UJI EFEKTIVITAS DAYA HAMBAT AIR PERASAN JERUK LEMON  (Citrus limon L.) TERHADAP PERTUMBUHAN Trichophyton rubrum  DENGAN METODE SUMURAN

Hannyfah Putri Wahyudi Wahyudi; Retno Sasongkowati; Suliati Suliati

Authors

Hannyfah Putri Wahyudi Wahyudi Teknologi Laboratorium Medis, Politeknik Kesehatan Kementerian Kesehatan Surabaya
Retno Sasongkowati Teknologi Laboratorium Medis, Politeknik Kesehatan Kementerian Kesehatan Surabaya
Suliati Suliati Teknologi Laboratorium Medis, Politeknik Kesehatan Kementerian Kesehatan Surabaya

Keywords:

Jeruk Lemon, Trichophyton Rubrum, Antijamur, Metode Sumuran, Zona Hambat

Abstract

ABSTRACT

Lemon (Citrus limon L.) is a fruit that contains various bioactive compounds, including flavonoids, saponins, tannins, limonene, and citric acid, which have been reported to possess antifungal properties. Trichophyton rubrum is a dermatophyte that is the primary causative agent of fungal infections affecting human skin, hair, and nails. Although the antifungal activity of lemon extracts has been widely reported, information regarding the effectiveness of fresh lemon juice against Trichophyton rubrum remains limited, highlighting the need for scientific investigation to evaluate its potential as a natural antifungal agent. This study aimed to determine the ability of fresh lemon juice (Citrus limon L.) to inhibit the growth of Trichophyton rubrum in vitro. This laboratory experimental study employed the well diffusion method on Sabouraud Dextrose Agar (SDA), with the diameter of the inhibition zone as the primary outcome variable. The results demonstrated that none of the treatments produced an inhibition zone, with a mean inhibition zone diameter of 0 mm, indicating that fresh lemon juice exhibited no antifungal activity against the growth of Trichophyton rubrum. These findings suggest that fresh lemon juice is ineffective as an antifungal agent against Trichophyton rubrum when tested using the well diffusion method. This study provides a scientific basis for future research focusing on the development of more effective extraction techniques or formulations with higher concentrations of bioactive compounds to enhance the antifungal potential of lemon.

ABSTRAK

Jeruk lemon (Citrus limon L.) merupakan salah satu buah yang mengandung senyawa bioaktif, seperti flavonoid, saponin, tanin, limonen, dan asam sitrat, yang dilaporkan memiliki aktivitas antijamur. Trichophyton rubrum merupakan dermatofita penyebab utama infeksi kulit, rambut, dan kuku pada manusia. Meskipun aktivitas antijamur ekstrak jeruk lemon telah banyak dilaporkan, informasi mengenai efektivitas air perasan segar terhadap Trichophyton rubrum masih terbatas sehingga diperlukan pengujian untuk memperoleh bukti ilmiah mengenai potensinya sebagai antijamur alami. Penelitian ini bertujuan untuk mengetahui kemampuan air perasan jeruk lemon (Citrus limon L.) dalam menghambat pertumbuhan jamur Trichophyton rubrum secara in vitro. Penelitian ini merupakan penelitian eksperimental laboratorium menggunakan metode difusi sumuran pada media Sabouraud Dextrose Agar (SDA), dengan variabel utama berupa diameter zona hambat yang terbentuk di sekitar sumuran. Hasil penelitian menunjukkan bahwa seluruh perlakuan tidak menghasilkan zona hambat dengan diameter rata-rata sebesar 0 mm, sehingga air perasan jeruk lemon tidak menunjukkan aktivitas antijamur terhadap pertumbuhan Trichophyton rubrum. Temuan ini mengindikasikan bahwa air perasan jeruk lemon dalam bentuk segar belum efektif digunakan sebagai agen antijamur terhadap Trichophyton rubrum menggunakan metode sumuran. Penelitian ini memberikan kontribusi ilmiah sebagai dasar bagi pengembangan penelitian lanjutan melalui penggunaan metode ekstraksi yang lebih optimal atau formulasi dengan konsentrasi senyawa aktif yang lebih tinggi untuk meningkatkan potensi antijamur jeruk lemon.

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References

Aboody, M. S. Al, & Mickymaray, S. (2020). Anti-fungal efficacy and mechanisms of flavonoids. In Antibiotics (Vol. 9, Number 2). MDPI AG. https://doi.org/10.3390/antibiotics9020045

Arya, K., Chaturvedi, S., Usmani, S. A., Chandra, S., Bhardwaj, N., Kumar, M., ... & Singh, A. (2025). Dermatophytosis in context of Trichophyton rubrum: host defense mechanisms, virulence factors, and treatment innovations. Molecular Biology Reports, 52(1), 634. https://doi.org/10.1007/s11033-025-10744-4

Barac, A., Stjepanovic, M., Krajisnik, S., Stevanovic, G., Paglietti, B., & Milosevic, B. (2024). Dermatophytes: update on clinical epidemiology and treatment. Mycopathologia, 189(6), 101. https://doi.org/10.1007/s11046-024-00909-3

Bento-Oliveira, A., Starosta, R., & de Almeida, R. F. M. (2024). Interaction of the antifungal ketoconazole and its diphenylphosphine derivatives with lipid bilayers: Insights into their antifungal action. Archives of Biochemistry and Biophysics, 753, 109919. https://doi.org/10.1016/J.ABB.2024.109919

Chen, X. F., Ding, Y. Y., Guan, H. R., Zhou, C. J., He, X., Shao, Y. T., ... & Chen, S. H. (2024). The pharmacological effects and potential applications of limonene from Citrus plants: A review. Natural Product Communications, 19(5), 1934578X241254229. https://doi.org/10.1177/1934578X241254229

Dellière, S., Jabet, A., & Abdolrasouli, A. (2024). Current and emerging issues in dermatophyte infections. PLoS pathogens, 20(6), e1012258. https://doi.org/10.1371/journal.ppat.1012258

García-González, G., González, G. M., & Bonifaz, A. (2025). Antifungal Resistance in Dermatophytes: An Emerging Threat to Global Public Health. Current Fungal Infection Reports, 19(1), 9. https://doi.org/10.1007/s12281-025-00508-9

Gupta, A. K., Mann, A., Polla Ravi, S., & Wang, T. (2024). An update on antifungal resistance in dermatophytosis. Expert Opinion on Pharmacotherapy, 25(5), 511-519. https://doi.org/10.1080/14656566.2024.2343079

Gupta, A. K., Wang, T., Mann, A., Piguet, V., Chowdhary, A., & Bakotic, W. L. (2025). Mechanisms of resistance against allylamine and azole antifungals in Trichophyton: A renewed call for innovative molecular diagnostics in susceptibility testing. PLoS pathogens, 21(2), e1012913. https://doi.org/10.1371/journal.ppat.1012913

Gupta, A. K., Wang, T., Mann, A., Polla Ravi, S., Talukder, M., Lincoln, S. A., ... & Bakotic, W. L. (2024). Antifungal resistance in dermatophytes–review of the epidemiology, diagnostic challenges and treatment strategies for managing Trichophyton indotineae infections. Expert Review of Anti-Infective Therapy, 22(9), 739-751. https://doi.org/10.1080/14787210.2024.2390629

Hapsari, D. L. (2024). Perbandingan Metode Difusi Cakram dan Sumuran Pada Uji Daya Hambat Perasan Jeruk Lemon (Citrus limon L.) terhadap Pertumbuhan Jamur Candida albicans. http://repo.poltekkes-surabaya.ac.id/id/eprint/8935

Kruithoff, C., Gamal, A., McCormick, T. S., & Ghannoum, M. A. (2023). Dermatophyte infections worldwide: increase in incidence and associated antifungal resistance. Life, 14(1), 1. https://doi.org/10.3390/life14010001

Langfeldt, A., Gold, J. A., & Chiller, T. (2022). Emerging fungal infections: from the fields to the clinic, resistant Aspergillus fumigatus and dermatophyte species: a one health perspective on an urgent public health problem. Current Clinical Microbiology Reports, 9(4), 46-51. https://doi.org/10.1007/s40588-022-00181-3

Moskaluk, A. E., & VandeWoude, S. (2022). Current topics in dermatophyte classification and clinical diagnosis. Pathogens, 11(9), 957. https://doi.org/10.3390/pathogens11090957

Ortiz, B., Ballesteros?Monrreal, M. G., Rosales?Tamashiro, J., Bush, M., Salmanton?García, J., & Fontecha, G. (2024). Global insights and trends in research on dermatophytes and dermatophytosis: a bibliometric analysis. Mycoses, 67(10), e13803. https://doi.org/10.1111/myc.13803

Poirier, W., Faway, É., Yamada, T., Ozawa, K., Maréchal, F., Salamin, K., ... & Mignon, B. (2025). Optimisation of a murine infection model with Trichophyton mentagrophytes for studying the pathogenesis of dermatophytosis. Mycoses, 68(12), e70141. https://doi.org/10.1111/myc.70141

Silva, E. P., Rocha, M. A. N. d., Silva, R. N. M., Moura-Mendes, J., Sousa, G. R. d., de Souza-Ferrari, J., Barbosa-Filho, J. M., Lima, E. d. O., & Pereira, F. d. O. (2023). Antifungal and Antibiofilm Activity of Riparin III against Dermatophytes. Journal of Fungi, 9(2), 231. https://doi.org/10.3390/jof9020231

Silva, L. B. B. d., Oliveira, L. S. d., Furlan, T. B. P., Almeida, B. C. T., Garcia, M. T., Song, Y., Peres, N. T. d. A., & Carmo, P. H. F. d. (2026). Dermatophytosis: An Update on Global Epidemiology. Journal of Fungi, 12(7), 503. https://doi.org/10.3390/jof12070503

Wahyu, F. F., Putra, C. B. P. E., & Sayoeti, M. F. W. (2023). Resistensi Anti Jamur Pada Dermatofitosis: Review Literatur. Jurnal Ilmu Kedokteran Dan Kesehatan, 10. https://doi.org/10.33024/jikk.v11i5.13947

Widhiastuti Fitriani, Handamari Dhyah Aksarani, & Musy Rahajeng. (2023). Studi Retrospektif Kunjungan Pasien Baru Mikosis Superfisialis di Poliklinik Kulit dan Kelamin RSUD Dr. Soedono Madiun, Indonesia Januari-Desember 2021 (Vol. 50, Number 4). https://doi.org/doi:10.55175/cdk.v50i4.853

Wiederhold, N. P. (2022). Emerging fungal infections: new species, new names, and antifungal resistance. Clinical chemistry, 68(1), 83-90. https://doi.org/10.1093/clinchem/hvab217

Zahrani, N. A. (2024). Perbandingan metode difusi disk dan difusi sumuran pada uji antijamur ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan jamur Trichophyton rubrum. http://repo.poltekkes-surabaya.ac.id/id/eprint/8950

Zulfah, I. T. (2024). Kadar hambat minimum (KHM) dan kadar bunuh minum (KBM) pada ekstrak daun jeruk purut (citrus hystrix DC) terhadap jamur Trichophyton Rubrum (Doctoral dissertation, Politeknik Kesehatan Kemenkes Surabaya). http://repo.poltekkes-surabaya.ac.id/id/eprint/8847