PEMBERIAN BORAKS DENGAN TIGA VARIASI DOSIS TERHADAP AKTIVITAS ENZIM SGOT DAN SGPT DALAM DARAH MENCIT JANTAN MELALUI PROSES INDUKSI
DOI:
https://doi.org/10.51878/cendekia.v7i1.16547Keywords:
Boraks, SGPT, SGOT, Mencit JantanAbstract
The misuse of borax as an unauthorized food additive is still widely found in processed foods to improve texture and shelf life. This study aims to analyze the effect of subacute oral borax administration across three dose variations on the activity of Serum Glutamic Pyruvic Transaminase (SGPT) and Serum Glutamic Oxaloacetic Transaminase (SGOT) in male mice (Mus musculus). This true experimental study employed a post-test only control group design using 32 male Balb/c mice divided into four groups: control, treatment 1 (1.51 mg/kg BW), treatment 2 (3.02 mg/kg BW), and treatment 3 (4.52 mg/kg BW) administered orally for 14 days. The One-Way ANOVA test showed significant differences among groups for both SGPT activity (F(3, 28) = 71.957; p < 0.001) and SGOT activity (F(3, 28) = 37.983; p < 0.001). Post-hoc Tukey Honestly Significant Difference (HSD) tests confirmed that SGPT levels increased significantly across escalating dose groups (p < 0.001), whereas SGOT levels between treatment groups 2 and 3 showed no statistically significant difference (p = 0.094). The increased activity of these transaminase enzymes indicates altered hepatocyte membrane permeability and supports the presumption of dose-dependent hepatocellular injury induced by subacute borax exposure.
ABSTRAK
Penyalahgunaan boraks sebagai bahan tambahan pangan non-pangan masih sering ditemukan di masyarakat untuk mengawetkan dan memperbaiki tekstur makanan olahan. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian boraks secara subakut dalam tiga tingkatan dosis terhadap aktivitas enzim Serum Glutamic Pyruvic Transaminase (SGPT) dan Serum Glutamic Oxaloacetic Transaminase (SGOT) pada mencit jantan (Mus musculus). Penelitian eksperimental murni (true experimental) ini menerapkan rancangan post-test only control group design menggunakan 32 ekor mencit jantan galur Balb/c yang terbagi ke dalam empat kelompok, yaitu kontrol, perlakuan 1 (1,51 mg/kg BB), perlakuan 2 (3,02 mg/kg BB), dan perlakuan 3 (4,52 mg/kg BB) dengan pemberian per oral selama 14 hari. Hasil uji One-Way ANOVA menunjukkan perbedaan yang sangat signifikan antarkelompok perlakuan, baik pada enzim SGPT (F(3, 28) = 71,957; p < 0,001) maupun SGOT (F(3, 28) = 37,983; p < 0,001). Uji lanjut Tukey Honestly Significant Difference (HSD) mengonfirmasi bahwa aktivitas SGPT meningkat nyata antarkelompok perlakuan dosis bertingkat (p < 0,001), sedangkan pada SGOT peningkatan antara kelompok perlakuan 2 dan 3 tidak berbeda bermakna secara statistik (p = 0,094). Peningkatan aktivitas enzim transaminase ini mengindikasikan adanya gangguan permeabilitas membran hepatosit dan mendukung dugaan terjadinya cedera hepatoseluler subakut yang berkorelasi dengan kenaikan dosis paparan boraks.
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